Fast, robust and precise 3D localization for arbitrary point spread functions
Published in bioRxiv, 2017
We present a fitter for 3D single-molecule localization of arbitrary, experimental point spread functions (PSFs) that reaches minimum uncertainty for EMCCD and sCMOS cameras,and achieves more than 105fits/s. We provide tools to robustly model experimental PSFsand correct for depth induced aberrations, which allowed us to achieve an unprecedented3D resolution with engineered astigmatic PSFs, and acquire high quality 3D superresolution images even on standard microscopes without 3D optics.